FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4009358
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT70900.044.0024035
GTAGCTG71300.043.6237536
TACTCGG15850.042.3892673
CGAGTAG20050.041.9457363
GAGTAGC47050.041.8411454
CCGAGTA20950.041.7131162
CTACTCG16100.041.438712
TTGGGAG108850.040.4019176
GCTGGGA131650.039.9706239
TAGCTGG77000.039.539217
GCACTTT86450.037.237721
GGAGGCT105000.036.9602139
CCCGAGT25600.036.3481861
AAAGTGC65950.035.763344
ACTTTGG92450.035.3713383
AAGTGCT67100.035.3609125
CGGAGGT22250.035.263897
TTTGGGA104700.034.9130255
CCCAAAG84800.034.2491761
CACTTTG95000.033.48182
Produced by FastQC (version 0.11.5)