FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714274
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9550.038.8618772
CGAGTAG9450.038.278863
TTGGGAG63350.036.9282576
CTACTCG7350.036.4323842
AGTAGCT36050.035.7063455
GCTACTC20550.034.580081
TACTCGG7900.034.4906233
GTAGCTG36200.034.2587436
GCTGGGA68450.034.1088459
GAGTAGC24200.032.8073734
GGAGGCT58300.032.6340189
CCCGAGT13300.032.553451
GCACTTT52850.031.7895261
CGGAGGT12750.031.6857767
TTTGGGA62900.031.5182365
TAGCTGG41400.031.4308057
GGGAGGC83250.030.6966328
TGGGAGG80650.029.5893527
TAGCGCG804.7953465E-429.3616285
CGCCGTA1452.5860572E-829.26047794
Produced by FastQC (version 0.11.5)