FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033079
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10550.041.9218642
CGAGTAG9900.040.3963133
TACTCGG8450.039.532993
GAGTAGC25050.036.4323774
TTGGGAG65050.035.910036
CTACTCG8500.035.4263342
GTAGCTG40850.035.207576
AGTAGCT40900.035.0808455
CCCGAGT14800.035.0012361
GCTGGGA78700.034.2171339
TCGCGTT1405.0567905E-1033.6020285
TAGCTGG46100.033.5418747
GGGAGGC80800.031.0614878
CGGAGGT11000.030.7632457
CCCAAAG55600.030.1527981
GCACTTT57300.029.9156991
GGAGGCT57850.029.8956629
TTTGGGA67650.029.1366585
TGGGAGG83100.029.127197
AAAGTGC43950.029.1141134
Produced by FastQC (version 0.11.5)