FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8559847
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG43850.074.5995253
CCGAGTA45450.073.544152
TACTCGG53800.067.42723
GAGTAGC112550.066.836634
AGTAGCT183000.066.319645
GTAGCTG189200.064.0118266
GCTACTC132400.063.3997731
CTACTCG55650.063.267792
GCTGGGA412600.063.0556839
CCCGAGT58350.061.3882641
TAGCTGG214150.057.8913157
GGAGGCT327400.057.4214749
TTGGGAG268500.054.8955086
AAAGTGC253200.054.769164
AAGTGCT258550.054.546615
CCCAAAG305900.053.3605161
CCAAAGT279200.053.018842
AGTGCTG264550.052.8524136
GTGCTGG266800.052.812217
TCGGGAG74300.052.4209826
Produced by FastQC (version 0.11.5)