FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8559847
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG42850.077.0769353
TACTCGG63300.072.986213
CCGAGTA46800.072.6103062
CTACTCG63350.071.420982
GAGTAGC109700.070.075824
GCTACTC145350.069.006451
AGTAGCT182000.066.503885
GCTGGGA416200.065.149689
GTAGCTG187600.064.756156
CCCGAGT58550.063.914431
TCGGGAG76100.062.5496446
CTCGGGA75850.060.4066625
GGAGGCT342750.059.9877179
TAGCTGG212350.059.545437
AAAGTGC252450.058.7842674
AAGTGCT256500.058.6748125
ACTCGGG78300.058.5978164
TTGGGAG272550.058.0914346
CCCAAAG306500.056.7904551
CCAAAGT289000.055.4888272
Produced by FastQC (version 0.11.5)