FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3663291
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15000.043.856233
CCGAGTA15650.042.9342962
TTGGGAG90550.039.6460046
CTACTCG12300.039.3472862
CGGAGGT18550.039.262367
AGTAGCT56150.038.8295865
TACTCGG12600.038.4114953
GCTACTC31500.038.0437621
GAGTAGC35700.037.9069484
GTAGCTG58650.037.1744466
GCTGGGA101900.036.844039
GGAGGCT86450.036.471379
GCACTTT72500.035.327451
TAGCTGG63350.035.231867
CCCGAGT21750.033.490861
TTTGGGA87750.033.4142725
GGGAGGC119550.033.4085738
GGAGGTT49200.032.4715088
ACTTTGG77450.032.3977133
CTCGGGA15450.032.238285
Produced by FastQC (version 0.11.5)