FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513202
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG3600.041.882831
ACGTCGA2000.032.982731
GTCGAGA6600.022.8409442
AGTAGCT54450.022.0616475
CCGAGTA14450.021.8431362
CGAGTAG13450.021.715263
GCTGGGA96400.021.4483789
GTAGCTG57600.021.3800586
GAGTAGC35000.020.862164
CTACTCG11500.020.4823682
TAGCTGG63350.019.7312167
TTGGGAG63950.019.4040996
TACTCGG12250.019.2277973
GCTACTT23850.018.768251
GGAGGCT67850.018.6996179
CTACTTG22450.018.6759052
GGGAGGC84750.017.6321898
TCGGGAG16450.017.4298656
CTCGGGA14900.017.3888975
AAAGTGC51400.017.3218524
Produced by FastQC (version 0.11.5)