FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995484
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10250.038.9819223
CCGAGTA10400.038.8762252
AGTAGCT41200.036.738965
GTAGCTG41850.035.7190486
GAGTAGC27000.035.168664
GCTGGGA77800.031.9536919
TAGCTGG47350.031.6682767
CGTCGAG1351.2934834E-831.4197241
CCCGAGT14550.030.4479791
TTGGGAG57050.029.3334086
TACTCGG8450.027.2588523
TTGCGCG350.00883771626.85790478-79
GGAGGCT58000.026.7381239
CTACTCG8500.026.5485782
GCTACTC21200.026.4548131
GGGAGGC74700.026.2966618
TGGGAGG77500.024.746417
CGCGAGA1151.3524186E-424.5893481
CCCAAAG55600.024.2429161
GCACTTT51100.023.9798281
Produced by FastQC (version 0.11.5)