FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459956
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7900.032.751063
TTGGGAG62150.031.7606816
TCGCGTT753.26264E-431.3566325
CGAGTAG10150.030.1256983
AGTAGCT41050.030.019875
GAGTAGC26500.029.9958744
GCTGGGA73650.029.2250699
CTACTCG7750.029.1380652
CCGAGTA11050.028.9512842
GGGAGGC75850.028.9362958
GTAGCTG42550.027.6135396
GGAGGCT58900.027.5274899
TGGGAGG78800.027.0180247
CGGAGGT10200.026.7244937
CCCGAGT14900.026.2390041
TCGGGAG12200.026.1957446
CCGGGTT8800.025.6354058
TAGCTGG46000.025.4403537
CGGGTTC8500.025.433249
GCACTTT55700.024.439831
Produced by FastQC (version 0.11.5)