FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3158901
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13000.033.331292
CGAGTAG13050.032.8411143
TTGGGAG59250.032.681446
AGTAGCT43850.032.3278665
CTACTCG8150.031.7843072
TACTCGG8050.031.5925643
GCTGGGA77650.031.4686839
GAGTAGC28600.031.12264
GTAGCTG45750.030.7165476
CCCGAGT15700.030.6092661
GGAGGCT60450.030.2391769
CGTACCG650.006094509728.9823533
TAGCTGG51000.027.918317
GGGAGGC76950.027.6634458
GCTACTC23000.026.4249571
TCGCGTT909.40914E-426.164215
CCCAAAG55850.025.3075731
TGGGAGG81100.025.1469637
TTTGGGA64550.025.0252275
GCACTTT55100.024.796981
Produced by FastQC (version 0.11.5)