FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3158901
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89440.28313644523839143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11250.038.4457442
CTACTCG10300.036.0580982
TACTCGG9950.035.9084433
CGGAGGT14100.035.6357847
AGTAGCT43000.035.3117565
CGAGTAG11900.034.764963
GAGTAGC27900.034.205114
GTAGCTG44500.033.9887476
TTGGGAG63700.033.84646
GCTGGGA77500.032.5931369
CCCGAGT16000.031.7572081
GGAGGCT63200.031.4245369
GCTACTC24700.031.2381571
TAGCTGG49500.030.7369987
CTCGGGA13500.030.2949875
GGAGGTT36200.028.9233638
GGCGGAG22550.028.3516035
ACTCGGG14200.028.1403644
GGGAGGC86300.028.0731688
CCCAAAG57250.027.6944621
Produced by FastQC (version 0.11.5)