FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130198
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAG56800.032.023416
GCTGGGA69600.030.2504679
TACTCGG7800.030.1604983
CCGAGTA11500.029.8686712
CCCGAGT14100.029.7288911
GGAGGCT53600.029.7233819
AGTAGCT39350.029.1720225
GTAGCTG39650.029.0420866
GGGAGGC68300.028.7638248
CTACTCG7700.028.7208982
CGAGTAG11450.027.9425813
GAGTAGC27350.027.3502454
GCTACTC19850.026.8117871
TAGCTGG47100.026.2437527
CTCGGGA9850.025.7916375
GGAGGCC39650.025.0093579
CCCGAAT5100.024.9345421
CGGAGGT10950.024.8945877
TGGGAGG74300.024.7963837
CCCAAAG50150.024.7936691
Produced by FastQC (version 0.11.5)