FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130198
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10550.038.7673873
TTGGGAG61500.035.3716936
CTACTCG10250.035.3156052
CGGAGGT12700.033.6689227
GGAGGCT57600.033.6130529
GCTACTC22600.033.1053471
CCGAGTA11250.032.5943152
GGGAGGC77250.032.2390948
CTCGGGA12600.032.0853545
TCGGGAG12800.031.9344336
CGAGTAG10750.031.9237693
CCCGAGT14300.031.91871
GCTGGGA70750.031.8157569
AGTAGCT37250.030.2875335
TGGGAGG78850.029.7961317
GTAGCTG38150.029.5574286
GCACTTT54550.028.9837131
GCGGAGG14950.028.2846786
GGCGGAG18050.028.1271325
ACTCGGG14600.028.0134224
Produced by FastQC (version 0.11.5)