FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12399609
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG37750.057.7048723
TACTCGG45000.057.642733
CCGAGTA40900.057.539792
CTACTCG45850.055.4222762
CCCGAGT57000.050.3008881
GCTGGGA374500.049.3221479
GAGTAGC104100.049.191354
CGGAGGT65900.048.8188867
GCTACTC123700.048.3106581
GGAGGCT295050.046.24849
GTAGCTG176100.044.7223476
TCGGGAG63650.043.9120526
AGTAGCT180200.043.667615
CTCGGGA59250.043.5530365
TAGCTGG197500.042.454367
GCGGAGG80300.041.5680736
GTGCTGG264650.039.9638377
GGCGGAG95850.039.3697245
AGGCGGA96050.039.2179644
CCCAAAG346750.038.2448771
Produced by FastQC (version 0.11.5)