FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12399609
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG50800.061.7396933
CGAGTAG37300.059.1111373
CTACTCG52700.058.3692862
CCGAGTA40500.055.433472
GCTACTC132800.052.7530561
CGGAGGT72950.051.3533557
GCTGGGA387350.050.779329
GGAGGCT305200.049.713489
TCGGGAG67900.049.448176
CTCGGGA64500.048.9376955
GAGTAGC103800.048.74524
CCCGAGT55550.048.5144841
AGTAGCT177550.044.4260865
GCGGAGG90150.043.4139636
GTAGCTG182150.043.1937836
TAGCTGG195300.043.1678777
GTGCTGG276000.043.1487
AGGCGGA107600.043.03764
ACTCGGG73500.043.0365034
GGCGGAG110300.042.1663745
Produced by FastQC (version 0.11.5)