FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559542
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT602.5538611E-854.8438535
CTCGCGT902.5102054E-1047.0090184
CGAGTAG10750.046.7903753
CCGAGTA11450.045.9853132
CCCGAGT13950.042.557891
GAGTAGC26250.042.442434
AGTAGCT41400.040.9909555
GTAGCTG42200.039.211316
GCTGGGA74400.038.655879
TAGCTGG46400.037.9892357
TTGGGAG57700.036.6621486
ACTCGCG1055.1077222E-835.8163953
TACTCGG7000.035.592543
AAAGTGC37850.033.781914
CGTAACG702.1832866E-433.577874
CTACTCG7300.032.199852
CGGAGGT10900.031.9118887
CCCAAAG50850.031.7824211
GGAGGCT49800.031.7063929
TTTGGGA59650.031.2079985
Produced by FastQC (version 0.11.5)