FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559542
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT40900.042.039585
CCGAGTA10250.040.7920232
TTGGGAG63250.040.1066936
GTAGCTG41600.039.523776
GAGTAGC25200.039.5217254
GCTGGGA75950.038.101769
TACTCGG9750.038.0647623
CGAGTAG10200.037.7671553
TAGCTGG47050.036.842617
CTACTCG9250.036.5678832
CGGAGGT13150.036.0810557
GCTACTC22750.035.986471
TTTGGGA61450.035.6257975
GCACTTT53850.035.4741251
CCCGAGT13800.034.0951271
GGAGGCT63500.034.031129
ACTTTGG57650.033.4921573
CTTTGGG62300.033.1037374
CACTTTG59200.032.4571722
CCCAAAG49700.032.1879961
Produced by FastQC (version 0.11.5)