FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10477242
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA67200.06.503905394-95
GTCGTGT73850.06.36854794-95
GCGTCGT82350.06.230398794-95
CGTCGTG79800.06.19136194-95
AGCGTCG91350.06.05861194-95
CGTGTAG68000.05.798622694-95
GAGCGTC105850.05.767238694-95
GATCGGA161650.05.67201494-95
TAGATCG52950.05.562645494-95
ATCGGAA155500.05.545004492-93
TCGGAAG158300.05.506946694-95
CGGAAGA163100.05.446823694-95
GCTTACG18950.05.389450690-91
AGATCGG176750.05.37559194-95
TTACGGT17350.05.3393894-95
TACGGTG19150.05.3336694-95
AGAGCGT112700.05.100551692-93