FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120527
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT17500.06.108076694-95
AGCGTCG18850.06.0486794-95
ATCGGAA32850.05.857059594-95
TCGGAAG34600.05.56082194-95
GATCGGA33450.05.46795194-95
TCGTGTA15054.0017767E-115.366268692-93
CGTGTAG15104.3655746E-115.348499394-95
AGATCGG36300.05.30039792-93
TAGATCG11301.7277307E-75.254722690-91
AAGAGCG28000.05.17489894-95
CGTCGTG17909.094947E-125.042670294-95
GTCGTGT17004.7293724E-115.030180594-95