FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476078
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11200.040.3085333
CTACTCG11050.039.1533852
TTGGGAG66600.036.1224336
AGTAGCT39600.034.554965
GGAGGCT63900.034.049069
GCTGGGA77150.033.0741659
GTAGCTG40900.032.8567056
GAGTAGC28250.031.9609994
CCGAGTA11500.031.8963172
GGGAGGC88450.031.8770738
TAGCTGG44450.031.503827
CGGAGGT13650.030.6391077
TGGGAGG87200.029.8007667
TCGGGAG15600.029.2165136
TTTGGGA68250.029.212975
CTCGGGA14900.029.0344815
GCACTTT60350.028.9315681
CGAGTAG11650.028.6600023
CCCGAGT15250.027.4660051
ACTTTGG62600.027.4196763
Produced by FastQC (version 0.11.5)