FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707748
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7150.031.5643792
TTCGGCG753.2712202E-431.3422875
TCGCGTT600.00415615331.3422855
CGAGTAG9250.030.49523
GCTGGGA61650.029.3481779
TTGGGAG48850.028.7758776
TACTCGG7700.028.6965083
GTAGCTG36150.028.6112176
AGTAGCT36550.028.4267255
GGGAGGC59650.027.9686458
CCGAGTA10300.027.3889942
CCCGAGT13450.027.3233491
GCTACTC17150.026.9230081
TAGCTGG39900.026.6252277
CTCGGGA9700.025.687755
GGAGGCT47850.025.5355329
GAGTAGC24050.025.2171824
CCCAAAG44300.025.2061331
GCGAGTA950.001286545324.7975061
CGGAGGT7400.024.7736877
Produced by FastQC (version 0.11.5)