FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707748
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8250.039.8635753
CGAGTAG8550.037.9153563
CTACTCG8150.036.8938942
CCGAGTA9450.036.2930262
CGGAGGT10000.035.7057157
GAGTAGC21600.033.931494
TCGCGTT702.190685E-433.558625
GCTGGGA63450.033.5421339
GGAGGCT52450.033.2316979
AGTAGCT34500.033.2278985
TTGGGAG51700.031.9872026
GTAGCTG34550.031.9553786
GCTACTC21700.031.8606851
CTCGGGA10500.031.3213795
CCCGAGT13200.030.9986551
GGAGGTT28450.030.7153028
TCGGGAG12250.030.298086
TAGCTGG38550.029.8583497
GGGAGGC69450.029.8325548
GGCGGAG13900.029.4060445
Produced by FastQC (version 0.11.5)