FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7139470
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG29550.055.1950073
CTACTCG29600.054.0848432
CCGAGTA28250.051.874322
CGAGTAG27050.051.1948973
GCTACTC73450.050.1879651
GAGTAGC63200.047.9136734
GGAGGCT173550.047.734449
GCTGGGA221700.047.7223249
CGGAGGT39550.046.0634657
AGTAGCT108800.045.085915
TCGCGTT2750.044.761925
CCCGAGT40250.044.1079441
TTGGGAG159150.044.0095636
GTAGCTG109900.043.626586
CTCGGGA37850.042.115825
TAGCTGG123950.041.5614137
TCGGGAG40100.040.8271146
GGGAGGC214350.040.1990668
GTGCTGG146850.038.936167
CCCAAAG185750.038.7618261
Produced by FastQC (version 0.11.5)