FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7139470
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG29300.059.0223123
CCGAGTA26000.058.2281722
CGAGTAG25050.056.7520753
CTACTCG29750.056.4738042
GCTACTC76900.052.905181
GGAGGCT182100.051.40479
CCCGAGT34550.051.127781
GCTGGGA226700.050.4946029
TTGGGAG176250.049.4448136
GAGTAGC64000.049.138454
AGTAGCT108900.048.3756035
GTAGCTG108350.048.0538336
CTCGGGA35200.045.6329355
TCGGGAG38450.045.457826
TAGCTGG120250.045.243257
CGGAGGT41600.044.5307277
GGGAGGC237150.044.0135358
CCCAAAG186150.041.760281
AAAGTGC154550.041.055394
ACTCGGG41100.040.87914
Produced by FastQC (version 0.11.5)