FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213702
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10700.036.0230453
CCGAGTA11400.035.8794562
TACTCGG9850.033.8822673
CTACTCG9300.033.8706362
AGTAGCT41950.033.2793465
GAGTAGC26300.032.8861054
TTGGGAG59850.031.2585816
GTAGCTG43250.031.0835256
CCCGAGT15250.030.5908581
TAGCTGG47600.029.9193537
GCTGGGA74450.029.6026179
GGGAGGC76150.028.8800478
GCTACTC23750.028.5709881
GGAGGCT56250.027.8191389
CTCGGGA11550.027.6743415
TGGGAGG76400.026.3924967
CGTCGCA909.5146947E-426.1142675
GGAGGTT28250.025.9494468
CGGAGGT11050.025.9468177
GCACTTT54750.025.3040221
Produced by FastQC (version 0.11.5)