FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213702
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11250.038.0030333
CGGAGGT13150.036.080937
CCGAGTA12250.035.284272
TTGGGAG64600.033.6690256
TCGCGTT1405.12955E-1033.5573655
TACTCGG10050.033.19113
CTACTCG9650.032.619472
GTAGCTG41750.032.292936
AGTAGCT42500.031.9466085
GCTGGGA76000.031.895179
GGAGGCT63250.031.7143769
GAGTAGC28000.031.0410424
TAGCTGG44850.030.4798367
GGCGGAG19450.029.9514585
CCCGAGT16250.029.5346761
GGGAGGC84400.028.9986218
AGGCGGA19150.028.949154
CGCCGTA2151.8189894E-1228.49849194
CTCGGGA12850.028.1516235
GCACTTT53650.027.9773371
Produced by FastQC (version 0.11.5)