FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456358
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27900.065.588343
CCGAGTA28750.065.3872762
GAGTAGC67250.057.34324
CTACTCG30700.057.031682
TACTCGG30600.056.791013
GCTACTC80550.056.121131
AGTAGCT109900.055.9866685
GCTGGGA229550.055.3211449
GTAGCTG110850.054.288146
CCCGAGT36200.053.009131
GGAGGCT193850.051.6661539
TTGGGAG181350.050.5541536
TAGCTGG123350.049.5832257
CCCAAAG191000.046.7882581
CGGAGGT40900.046.542857
TCGGGAG39850.045.6118776
AAAGTGC155500.045.3737034
AAGTGCT160150.045.0915265
CTCGGGA38200.044.6885225
CCAAAGT175950.044.55242
Produced by FastQC (version 0.11.5)