FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456358
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28500.068.0777053
CCGAGTA29100.067.518012
TACTCGG31550.064.804863
CTACTCG32150.063.02252
GAGTAGC70350.059.9591264
GCTACTC82300.059.842241
GCTGGGA244300.059.5901039
AGTAGCT112500.059.107425
CCCGAGT36200.059.0342451
GGAGGCT206050.057.0639959
GTAGCTG116600.056.607326
TTGGGAG189450.056.2299276
TAGCTGG128900.054.5872237
CTCGGGA41750.053.9728855
TCGGGAG45300.051.6403856
AAAGTGC160100.051.1979564
AAGTGCT161050.051.1632165
CCCAAAG192150.050.591071
GTGCTGG161700.049.7416957
AGTGCTG162700.049.662786
Produced by FastQC (version 0.11.5)