FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222838
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG51100.071.3194663
CCGAGTA55450.067.060442
TACTCGG66950.066.286193
CTACTCG70200.062.9319342
GCTGGGA523500.060.878729
GCTACTC178450.060.13141
GAGTAGC136300.059.926164
CCCGAGT69950.058.897011
AGTAGCT231400.056.9698835
GTAGCTG235350.055.676576
GGAGGCT421400.055.4101529
TCGGGAG89100.053.3204426
TAGCTGG262500.052.5519337
AAAGTGC380650.052.2229654
CCCAAAG457300.052.1112671
CGGAGGT92900.052.076997
CTCGGGA86850.051.550815
GTGCTGG376400.051.377187
AGTGCTG387700.051.130296
AAGTGCT395900.050.6692475
Produced by FastQC (version 0.11.5)