FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222838
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG74350.070.346323
CGAGTAG50850.067.821783
CTACTCG77600.067.053832
CCGAGTA54850.065.204772
GCTGGGA536450.063.0314989
GCTACTC184850.062.3485261
GAGTAGC131900.061.2123834
CCCGAGT70800.058.218641
GGAGGCT431000.057.69759
AGTAGCT228850.057.6342855
CTCGGGA91700.056.668675
GTAGCTG233450.056.1433036
TCGGGAG96850.056.0055586
ACTCGGG93900.055.55684
AAAGTGC383000.054.9221954
GTGCTGG385650.053.9894757
AGTGCTG390700.053.9153986
AAGTGCT394600.053.80075
CCCAAAG460550.053.728641
TAGCTGG258200.053.0219657
Produced by FastQC (version 0.11.5)