FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8426815
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA41650.071.075632
CGAGTAG39950.068.969643
GAGTAGC105150.064.813744
AGTAGCT167400.062.8353845
TACTCGG46900.061.5984653
GTAGCTG170450.061.4870266
GCTGGGA363400.060.674369
CCCGAGT54450.060.6291581
GCTACTC113350.057.124421
CTACTCG46750.057.057822
TAGCTGG193900.056.5778357
GGAGGCT278800.053.1573839
TCGGGAG61100.050.6153146
AAAGTGC237650.050.205394
CGGAGGT64100.049.934067
TTGGGAG245500.049.7146076
AAGTGCT244950.049.4625325
GTGCTGG239800.049.1449287
CCCAAAG287750.048.9470561
CTCGGGA59000.048.318445
Produced by FastQC (version 0.11.5)