FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182011
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13250.043.6761743
CCGAGTA14100.042.3826182
TACTCGG10250.039.9347273
GAGTAGC31250.039.290214
CCCGAGT16950.038.9081041
AGTAGCT48400.037.906455
CTACTCG10250.037.6437722
GTAGCTG48250.037.307776
GCTGGGA87400.035.758069
TTGGGAG67200.034.7604186
TAGCTGG53650.034.4270827
GCTACTC23900.033.5067861
GGAGGCT65100.031.8362069
CTCGGGA14100.030.0273675
TCGGGAG15000.028.8266666
TTTGGGA67350.028.7775295
ACTCGGG14900.028.415164
GGGAGGC89600.028.3245588
AAAGTGC44650.027.4987474
CCCAAAG58200.027.1146221
Produced by FastQC (version 0.11.5)