FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7555111
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG61300.080.62653
CGAGTAG42500.080.253513
CTACTCG61650.079.646312
CCGAGTA45000.076.9385452
GAGTAGC105700.072.634914
AGTAGCT175450.070.938675
GCTGGGA418100.069.1570059
GTAGCTG181550.068.484276
AAAGTGC257700.065.653244
GGAGGCT355250.065.631789
AAGTGCT261150.065.5251855
CTCGGGA77200.065.354995
CCCGAGT55650.064.76841
TTGGGAG281150.064.732336
ACTCGGG76250.064.3124544
CCAAAGT283750.063.9342272
TCGGGAG82000.063.8837666
CCCAAAG309550.062.9808921
TAGCTGG205300.062.5674827
AGTGCTG272400.060.929026
Produced by FastQC (version 0.11.5)