FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402270
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG28700.060.401133
CCGAGTA26750.057.166562
CTACTCG29300.056.839932
CGAGTAG25500.055.8414343
GCTACTC70900.052.48861
GCTGGGA212000.051.3687369
CCCGAGT33300.051.321771
GGAGGCT171000.050.506479
GAGTAGC62150.050.3059234
AGTAGCT103150.049.9974755
TCGGGAG37050.049.7966776
CGGAGGT40200.049.744157
GTAGCTG106400.048.9939846
TAGCTGG118350.046.714187
CTCGGGA37700.046.3105625
TTGGGAG161250.045.5362556
ACTCGGG39650.044.5010834
GGGAGGC217500.041.8149388
GTGCTGG138800.041.1251037
CCCAAAG176450.040.4617271
Produced by FastQC (version 0.11.5)