FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3537728
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12900.047.099433
CCGAGTA13700.045.732042
GAGTAGC33550.038.0445484
GTAGCTG48600.037.910726
AGTAGCT49900.037.8494455
CCCGAGT18550.037.5923581
TAGCTGG55100.035.222377
GCTGGGA89500.035.1780629
TTGGGAG76250.032.1151586
GCGTCGT902.5515461E-531.4116341
CTACTCG10100.031.2494892
TACTCGG10800.030.0913013
GCTACTC24950.030.0267541
TCGCGTT1102.9222192E-629.9723665
GGAGGCT63950.028.8048539
AAAGTGC49000.028.1635384
GGGAGGC90750.028.1172838
AGCTGGG73800.027.6982968
GCACTTT65050.027.6692811
CCCAAAG62150.027.4441171
Produced by FastQC (version 0.11.5)