FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11090260
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG52550.061.811943
CCGAGTA42300.061.6765442
CTACTCG52800.060.296492
CGAGTAG41650.060.1580543
GCTGGGA387300.057.8338979
GAGTAGC107650.056.987654
GCTACTC134000.055.629321
CCCGAGT52150.054.9960331
AGTAGCT181450.054.0234155
GTAGCTG179600.053.20116
GGAGGCT315200.052.713629
TCGGGAG67400.050.383826
CTCGGGA64000.050.247755
TAGCTGG202300.049.4597637
CGGAGGT73400.049.2502177
CCCAAAG329700.048.0205421
GTGCTGG272150.047.747617
AAAGTGC274300.047.6963124
TTGGGAG282100.047.4418336
AGTGCTG277600.047.117826
Produced by FastQC (version 0.11.5)