FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230762
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12400.038.7118453
CCGAGTA13650.035.858572
AGTAGCT42950.034.947725
TTGGGAG63400.034.6200756
GTAGCTG44850.033.219816
GCTGGGA81400.032.3885739
GAGTAGC30000.031.839494
TAGCTGG49050.031.6195097
CCCGAGT17450.031.590331
GCTACTC21150.031.4103281
TACGCGG600.004135553731.3743023
GGGAGGC78900.031.0937948
GGAGGCT60600.030.0893219
TGGGAGG81650.028.6075337
TACTCGG9250.027.9824893
GCACTTT57250.027.981251
CCCAAAG56300.027.8676011
CGGAGGT12350.027.0191447
TTTGGGA66400.026.3612615
GGAGGCC46500.026.2768769
Produced by FastQC (version 0.11.5)