FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230762
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAG70050.037.024566
CGAGTAG11200.036.9453543
AGTAGCT43400.036.1858255
CTACTCG10800.036.1367872
CCGAGTA11500.035.9815642
CGGAGGT13800.035.7516487
TCGCGTT1451.8189894E-1135.670275
CGCGTTG1452.0008883E-1135.643776
GTAGCTG45150.035.3818176
GCTGGGA78250.035.254429
TACTCGG11250.035.1092833
TAGCTGG47300.034.9677247
GCTACTC26950.034.2318151
GGAGGCT66850.033.6036389
GGGAGGC89650.033.4937138
GAGTAGC28250.033.288944
CTCGCGT1807.2759576E-1231.3470884
CCCGAGT15000.031.0653741
CTCGGGA13050.030.625995
GCACTTT61750.030.3374311
Produced by FastQC (version 0.11.5)