FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7976945
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG44200.078.629473
CCGAGTA46500.077.4022
TACTCGG56600.072.786973
GAGTAGC110850.072.73024
AGTAGCT185150.070.2179645
GCTACTC136500.069.831211
CTACTCG57050.069.819342
GCTGGGA426450.069.174259
GTAGCTG191100.067.625336
CCCGAGT58100.064.480681
TAGCTGG215700.062.1398437
AAAGTGC275100.061.5927474
AAGTGCT284100.060.740565
GGAGGCT336700.060.3209889
CCAAAGT301500.060.098262
CCCAAAG329300.059.989521
AGTGCTG289900.059.2056856
TCGGGAG74050.058.671026
CTCGGGA73000.057.938465
GTGCTGG291050.057.850457
Produced by FastQC (version 0.11.5)