FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7976945
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG45200.078.611213
TACTCGG59250.076.0060353
CCGAGTA47750.075.774582
CTACTCG60150.074.110052
GCTACTC139900.073.375891
AGTAGCT184550.072.215865
GAGTAGC117750.072.1235664
GCTGGGA434150.070.2140059
GTAGCTG190550.070.060956
TAGCTGG210150.066.180287
CCCGAGT58500.065.3163151
AAAGTGC271050.063.960584
GGAGGCT342550.063.903169
AAGTGCT277900.063.4830365
CCAAAGT305400.061.5389822
CCCAAAG333900.061.2922481
AGTGCTG284000.060.6835826
TCGGGAG80400.059.8802076
ACTCGGG74100.059.4563264
CAAAGTG300850.059.4424933
Produced by FastQC (version 0.11.5)