FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10938443
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG65750.070.292523
CTACTCG64950.069.75442
CGAGTAG43850.068.600013
CCGAGTA47900.064.7661062
GCTACTC162500.064.147891
GCTGGGA440750.059.8437279
GGAGGCT377350.059.2015539
GAGTAGC115000.059.1278084
TCGGGAG85600.058.1687626
AGTAGCT190850.056.6437385
CTCGGGA83200.056.5066535
ACTCGGG85150.055.056644
CCCGAGT64150.054.2340161
GTAGCTG200450.053.456416
TTGGGAG292150.053.0165146
AAAGTGC310800.051.404874
GTGCTGG313450.050.81137
AAGTGCT322250.050.546875
CCCAAAG377350.050.4888421
TAGCTGG221650.050.080297
Produced by FastQC (version 0.11.5)