FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348858
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12950.029.465422
CGAGTAG13000.028.2615933
AGTAGCT48100.026.145995
GAGTAGC31200.025.8158784
GTAGCTG48100.025.7970096
GCTGGGA84400.025.2778199
TAGCTGG55000.024.0942047
TTGGGAG60950.023.0572746
CTACTCG10300.022.8681052
CCCGAGT18350.022.853641
GGAGGCT61900.022.7749189
TACTCGG10700.022.4507983
GCTACTC26400.021.4179921
GGGAGGC77850.019.9800058
CTCGGGA14250.019.5018625
CGCGAGT1951.107891E-519.331111
GCCGTAC1951.107891E-519.331111
TCGGGAG14200.019.197746
CCCGAAT5900.019.1672881
CCCAAAG58800.019.1523481
Produced by FastQC (version 0.11.5)