FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348858
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN94490.28215588717108936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13250.032.2963872
CGAGTAG13500.031.0002443
GCTGGGA84150.029.7454769
AGTAGCT48450.028.1444975
GTAGCTG49800.027.7329626
CCCGAGT17400.027.04821
TTGGGAG61200.026.9422536
GAGTAGC31450.026.3148484
TAGCTGG55450.025.7477557
TACTCGG11150.024.882043
GGAGGCT63700.024.4026159
GGGAGGC80350.022.9696568
CTACTCG10450.022.949982
CCCAAAG59300.022.4604991
AAAGTGC50050.022.1726074
GTGCTGG50450.021.7832187
TGGGAGG82250.021.7548667
GCACTTT52450.021.7148861
TTTGGGA61700.021.4146235
AAGTGCT52100.021.3894635
Produced by FastQC (version 0.11.5)