FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11317090
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG51900.078.561813
CCGAGTA57200.072.901172
TACTCGG71600.071.04473
CTACTCG71350.069.817832
GAGTAGC143350.068.243434
AGTAGCT229650.067.44435
GCTACTC178300.067.054311
GCTGGGA538550.066.339849
GTAGCTG243400.064.09156
TCGGGAG89350.061.026336
CCCGAGT72200.060.7477721
TAGCTGG268150.060.0411767
GGAGGCT434050.058.9957589
CTCGGGA91100.057.927555
AAAGTGC359550.057.535274
AAGTGCT373150.057.2763185
CCCAAAG426700.056.440851
GTGCTGG370900.056.0642667
CCAAAGT399050.055.9025042
AGTGCTG376250.055.1475836
Produced by FastQC (version 0.11.5)