FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11317090
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG57800.074.982513
CTACTCG70850.073.741112
TACTCGG74050.072.3358153
CCGAGTA63000.071.516222
GAGTAGC141400.070.2113954
GCTACTC177150.069.613031
AGTAGCT232250.068.877065
GCTGGGA546050.068.0545359
GTAGCTG243400.065.195086
CCCGAGT75300.063.089921
TCGGGAG97000.061.5342146
GGAGGCT448850.061.0912259
TAGCTGG267300.060.7411167
AAGTGCT369350.059.3665055
AAAGTGC362000.059.1872254
CCAAAGT395600.058.446182
CCCAAAG433600.058.3273121
AGTGCTG370950.058.317416
GTGCTGG374050.057.4577187
CTCGGGA95550.057.439585
Produced by FastQC (version 0.11.5)