FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8636724
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32450.071.059763
CCGAGTA37000.066.336432
TACTCGG44600.065.314853
CTACTCG45900.062.0577662
CCCGAGT44600.060.5529331
GAGTAGC87250.060.2582634
GCTGGGA327650.060.043889
GCTACTC111250.059.411341
AGTAGCT150350.056.8777245
GGAGGCT265050.056.734629
GTAGCTG151900.056.6214076
TCGGGAG55100.055.078296
CTCGGGA52450.054.6757975
TAGCTGG168250.053.0327727
AAAGTGC223050.052.412394
CCCAAAG280450.051.051451
ACTCGGG56500.050.6422424
AAGTGCT232850.050.594845
CGGAGGT60000.050.4676367
TTGGGAG230250.049.9737856
Produced by FastQC (version 0.11.5)