FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041339
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7600.032.788192
TACTCGG8500.030.4217823
AGTAGCT38900.030.2156015
GTAGCTG39700.029.8435766
GAGTAGC24400.029.288334
CGAGTAG9750.028.9326043
GCTGGGA69100.028.3585879
TAGCTGG42650.027.5534577
TTGGGAG55450.027.1324676
CCGAGTA11100.027.1089152
CGGAGGT10850.025.5609767
CCCGAGT13700.024.4231341
GGGAGGC68100.024.151698
GCTACTC20400.024.0251871
GGAGGCT53750.023.955149
TGGGAGG72550.022.8713847
GCACTTT46050.022.8212181
AGGCGGA14200.022.5144544
GTGCTGG42200.021.9436517
AAAGTGC41550.021.6124114
Produced by FastQC (version 0.11.5)