FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041339
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8750.036.5093573
CCGAGTA9950.033.995382
CTACTCG8550.033.51772
TACTCGG8900.033.2547653
CGGAGGT14050.032.4344027
GAGTAGC23000.030.8427054
TTGGGAG56850.030.5760756
CGCCGTA1551.4788384E-930.4024894
AGTAGCT37700.030.0321315
CCCGAGT13350.029.6060351
GCTGGGA71850.029.3582429
GGAGGCT55700.028.9300659
TCGCGTT650.006168727828.9105995
GTAGCTG38400.028.8729576
GCTACTC23000.028.8451981
AGGCGGA17400.028.6193644
GGCGGAG18150.028.472565
GCGGAGG15550.028.0971986
GGAGGTT34100.027.2785518
CTCGGGA11550.027.2523085
Produced by FastQC (version 0.11.5)