FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386650
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8050.045.548923
CGAGTAG9200.045.4759253
CTACTCG6950.044.6470832
CCGAGTA10600.044.353522
GAGTAGC21900.043.1450844
AGTAGCT34950.040.6199265
CCCGAGT13600.040.5374071
GTAGCTG36350.038.538186
GCTGGGA69500.038.0025379
GCTACTC18500.035.6586841
TAGCTGG40550.035.0051357
TTGGGAG56000.034.9208376
CCCAAAG48200.034.5093421
AAAGTGC36650.034.5030674
GCACTTT47850.033.8754841
CCAAAGT43950.033.5895962
AAGTGCT38900.032.7490775
GGAGGCT55200.032.352339
ACTCGGG11200.032.3185654
TTTGGGA53550.032.3048485
Produced by FastQC (version 0.11.5)