FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386650
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT751.4870966E-743.8471035
TACTCGG9150.042.6148643
CGAGTAG10050.042.5382353
TTGGGAG55750.040.6985636
AGTAGCT36900.040.6133165
GAGTAGC24550.040.568464
CCGAGTA10600.040.3319052
CTACTCG8750.040.2685932
GCTACTC20450.038.6555751
GCTGGGA72200.038.582859
AAAGTGC38900.038.0421524
CCAAAGT43100.037.9328272
AAGTGCT38850.037.6074145
GTAGCTG38750.037.5808686
CCCAAAG49050.037.3170051
TAGCTGG42500.037.028217
CCCGAGT13000.036.9192621
CAAAGTG44650.035.773513
GGAGGCT62150.035.6761259
CGGAGGT13150.035.3660287
Produced by FastQC (version 0.11.5)